Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

ANP32E promotes the proliferation and glycolysis of melanoma cells by regulating the Wnt/&beta:-catenin pathway

Yun Zhang¹, Yannan Jiang²

¹Department of Dermatology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu 225012, China; ²Department of Dermatology, The Fourth Affiliated Hospital of Nantong University, The First People’s Hospital of Yancheng, Yancheng, Jiangsu 224000, China.

For correspondence:- Yannan Jiang Email: jiangyannan0624@163.com Tel:+8651588508612

Accepted: 25 November 2021 Published: 30 December 2021

Citation: Zhang Y, Jiang Y. ANP32E promotes the proliferation and glycolysis of melanoma cells by regulating the Wnt/&beta:-catenin pathway. Trop J Pharm Res 2021; 20(12):2475-2480 doi: 10.4314/tjpr.v20i12.3

© 2021 The authors.
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Abstract

Purpose: This study aimed to explore the related molecular mechanism of acidic leucine-rich nuclear phosphoprotein 32 family member E (ANP32E) in melanoma cell proliferation and glycolysis.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assays were employed to determine mRNA and protein expression of ANP32E in four melanoma cell lines (A375, G361, SK-MEL-3, and A2058) and normal human immortalized keratinocytes (HACAT), while 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide cell proliferation and clone formation assays were used to detect cell proliferation of A375 cells after ANP32E knockdown. Enzyme-linked immunosorbent assay and western blotting were used to assess the role of ANP32E in glucose consumption and the levels of lactate, adenosine triphosphate (ATP), and glycolytic metabolism-related proteins. Protein expressions of β-catenin and c-Myc in melanoma cells after ANP32E knockdown were determined by Western blotting.

Results: Protein expression and mRNA levels of ANP32E were higher in the four melanoma cell lines than in normal human immortalized keratinocytes (p < 0.001). ANP32E suppression inhibited the proliferation of melanoma cells (p < 0.05). Additionally, ANP32E suppression decreased glucose consumption (p < 0.001), lactate (p < 0.05), ATP (p < 0.001), and glycolytic metabolism-related protein expression in melanoma cells (p < 0.05). Finally, ANP32E was confirmed to regulate Wnt/β-catenin signaling pathway (p < 0.05).

Conclusion: ANP32E promotes the proliferation and glycolysis of melanoma cells by regulating Wnt/β-catenin pathway, thus facilitating the potential discovery of advanced therapeutic targets in melanoma.

Keywords: ANP32E, Melanoma, Wnt, β-catenin, Cell proliferation, Glycolysis